| 2025 | This Ordinary Thing | Self (voice) |
| Janis Ian: Breaking Silence | Self |
| 2024 | The World According to Allee Willis | Self |
| An Unexpected Community | Self |
| Xuất Chúng: Cuộc Cách Mạng Hài Kịch | Self |
| 2023 | Bella! | Self |
| Carol Burnett: 90 Years of Laughter + Love | Self - Guest |
| Moving On | Evelyn |
| Hành Trình Theo Dấu Brady | Lou |
| 2022 | The Best of Netflix Is a Joke: The Festival | Self |
| Jane Fonda & Lily Tomlin: Ladies Night Live | Self |
| Stand Out: An LGBTQ+ Celebration | — |
| Still Working 9 to 5 | Self |
| The Martha Mitchell Effect | Self (archive footage) (uncredited) |
| 2021 | Live at Mister Kelly's | Self |
| Emily @ the Edge of Chaos | Ayn Rand (voice) |
| 2020 | Waging Change | Self |
| One World: Together at Home | Self |
| 2019 | Dolly Parton: Hành trình âm nhạc | Self |
| Still Laugh-In: The Stars Celebrate | Self |
| I Am Richard Pryor | Self - Actress and Comedian |
| 2018 | Người Nhện: Vũ Trụ Mới | Aunt May (voice) |
| TVTV: Video Revolutionaries | Self |
| Feminists: What Were They Thinking? | Self |
| Jane Fonda in Five Acts | Self - (archive footage) |
| 2015 | Grandma | Elle |
| 2014 | Altman | Self |
| 2013 | The Making of 'Nashville' | Self |
| Richard Pryor: Omit the Logic | Self |
| An Apology to Elephants | Narrator |
| Thú Nhận Với Tình Yêu | Susannah |
| Why We Laugh: Funny Women | Self |
| Inequality for All | Violet Newstead (archive footage) |
| 2012 | Stars in Shorts | Jason's Mom |
| The Procession | Jason's mom |
| 2011 | Vito | Self |
| 2009 | Điệp Vụ Báo Hồng 2 | Mrs. Berenger |
| 2007 | Cutting Edge Comedians of the '60s & '70s | Self (archive footage) |
| The Walker | Abigail Delorean |
| 2006 | Lạc Vào Thế Giới Kiến | Mommo (voice) |
| A Prairie Home Companion | Rhonda Johnson |
| 2005 | Saturday Night Live in the '80s: Lost and Found | Self |
| 2004 | Happy Birthday Oscar Wilde | Self |
| I ♥ Huckabees | Vivian Jaffe |
| Superstar in a Housedress: The Life and Legend of Jackie Curtis | Self |
| Goodnight, We Love You | Self |
| 2002 | Orange County | Charlotte Cobb |
| 2000 | The Kid | Janet |
| Altman on His Own Terms | Self |
| 1999 | Saturday Night Live: 25th Anniversary Special | Self |
| Tea with Mussolini | Georgina "Georgie" Rockwell |
| Get Bruce! | Self |
| 1998 | Krippendorf's Tribe | Ruth Allen |
| 1997 | Happy Birthday Elizabeth: A Celebration of Life | Self |
| 1996 | Edith Ann's Christmas (Just Say Noël) | Edith Ann (voice) |
| Getting Away with Murder | Inga Mueller |
| Flirting with Disaster | Mary Schlichting |
| The Celluloid Closet | Narrator (voice) |
| Kids for Character | Ms. Valerie Frizzle(archive footage) (voice) |
| 1995 | Blue in the Face | Waffle eater |
| Peter Allen: The Boy From Oz | Self |
| 1994 | Edith Ann: Homeless Go Home | Edith Ann (voice) |
| Sesame Street | All-Star 25th Birthday: Stars and Street Forever! | Ernestine (archive footage) |
| But... Seriously | Self (archive footage) |
| Edith Ann: A Few Pieces of the Puzzle | Edith Ann (voice) |
| 1993 | Sesame Street Stays Up Late! | Ernestine the Telephone Operator(uncredited) |
| The Beverly Hillbillies | Miss Jane Hathaway |
| Short Cuts | Doreen Piggot |
| And the Band Played On | Dr. Selma Dritz |
| Luck, Trust & Ketchup: Robert Altman in Carver Country | Self |
| An Evening with Lily Tomlin and Friends | Self / Ernestine / Judith Beasley / Mme Lupé / Others |
| 1992 | The Player | Lily Tomlin |
| 1991 | Shadows and Fog | Prostitute |
| The Search for Signs of Intelligent Life in the Universe | Trudy the Bag Lady / Agnus Angst / Kate / Brandy / Tina / Chrissy / Lud / Marie / Vince / Edie / Lynn / Marge |
| 1988 | Free to Be... a Family | Self |
| Big Business | Rose Ratliff / Rose Shelton |
| 1987 | Funny, You Don't Look 200: A Constitutional Vaudeville | Self |
| Lily Tomlin | Herself |
| 1986 | The Muppets: A Celebration of 30 Years | Self (archive footage) |
| Waiting Tables | — |
| 1984 | All of Me | Edwina Cutwater |
| 1982 | Lily for President? | Lily Tomlin / Various |
| 1981 | The Muppets Go to the Movies | Herself |
| Lily: Sold Out | Self / Various Characters |
| The Incredible Shrinking Woman | Pat Kramer / Judith Beasley / Telephone Operator |
| 1980 | Nine to Five | Violet Newstead |
| 1979 | Appearing Nitely | Self |
| 1978 | Moment by Moment | Trisha Rawlings |
| 1977 | The Paul Simon Special | Self |
| The Late Show | Margo |
| 1976 | TVTV Looks at the Oscars | Judy Beasley/Self |
| 1975 | Nashville | Linnea Reese |
| 1974 | Flip Wilson... Of Course | Self |
| 1973 | Juke and Opal | Opal |
| Lily | — |
| 1972 | Scarecrow in a Garden of Cucumbers | Telephone Voice (voice) (uncredited) |